A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Prakash, S.
- Effect of Aeration and Agitation on Volumetric Oxygen Transfer in Pichia pastoris Culture System
Authors
1 P.G. Department of Biotechnology, Udaya School of Engineering, Kanyakumari (T.N.), IN
Source
Asian Journal of Bio Science, Vol 4, No 1 (2009), Pagination: 63-68Abstract
The oxygen mass transfer coefficient (KLa) often serves to compare the efficiency of bioreactors and their mixing devices as well as being an important scale up factor. In submerged fermentation, four methods are available to estimate the overall oxygen mass transfer coefficient. Each method provides a distinct estimation of the value of KLa. Dynamic gassing out method was used to obtain a more probable value of KLa during the fermentation in 5L fermentor with Pichia pastoris. The aeration and agitation were varied to study the effect on KLa. Results showed that the KLa value increased with increase in agitation and aeration. While working in 5L fermentor with Pichia pastoris the maximum KLa value of 441.36 was obtained when the agitator speed 300 RPM and aeration 1.5 vvm was maintained. This shows that the volumetric oxygen transfer rate varies in variation with aeration and agitation and not based on organism used. The optimum conditions of agitation and aeration to achieve maximum KLa for a given microorganism depends on its shear sensitive nature.Keywords
KLa, Pichia pastoris, Aeration, Agitation, Dissolved Oxygen.
- Optimization and Comparative Analysis of Meat Infusion in Muller Medium for The Production of Effective Tetanus Toxin
Authors
1 Department of Biotechnology, Udaya School of Engineering, Udaya Nagar, Ammandivilai, Kanyakumari (T.N.), IN
Source
Asian Journal of Bio Science, Vol 4, No 2 (2010), Pagination: 226-229Abstract
Optimizations of fermentation medium for Clostridium tetani (MTCC 449) in the effective production of tetanus toxin were studied. Modified Muller Media with different concentration of Meat infusion has been used for the production of tetanus toxin . In this present work, the comparative study was carried out to determine the efficacy of tetanus toxin with the commercially prepared meat infusion broth compared and the meat infusion powder supplemented in Muller medium. The observed results showed the higher titer value of toxin in Muller media supplemented with meat infusion with higher concentration. The OD of the cell growth and pH changes due to buffering capacity were also studied. The better growth of the C.tetani was observed in the MM medium which contain higher rate of meat infusion broth. The level of the tetanus toxin was measured by Lf test. Minimal lethal dose was performed to check the presence of tetanus toxin.Keywords
Tetanus Toxin, Clostridium tetani, Fermentation Medium.- Marine Phytochemicals as a Source of Pharmacological Interest for Drug Resistant Methicillin Resistant Staphylococcus aureus (MRSA)
Authors
1 Department of Biotechnology, Udaya School of Engineering, Ammandivillai P.O., Kanyakumari (T.N.), IN
Source
Asian Journal of Bio Science, Vol 3, No 2 (2008), Pagination: 340-345Abstract
Antimicrobial drug resistance occurs in hospitals worldwide. One of the globally important micro-organisms is Methicillin Resistant Staphylococcus aureus (MRSA) which now causes more than 40% of all S. aureus bacteremias. Hence scientists are searching for natural compounds to control MRSA. Many marine organisms are known to produce bioactive compounds, but historically man has derived relatively few pharmaceutical agents from marine species. It is certainly true that most of the pharmacologically active products have been isolated from the sea especially from microalgae. In the present study a survey was made in the Nagercoil government hospital for studying bed sore infectious Staphylococcus aureus. From the samples collected, 20% were Staphylococcus spp. and 80% were other bacterial species. Of the 20% of Staphylococcus aureus, 33.4% Methicillin Resistant Staphylococcus aureus (MRSA). Different solvent extracts of four marine micro algae were screened against MRSA pathogens. The extraction of antimicrobials from marine micro algae reveals that n-Butanol gave maximum extraction. Among the total Four microalgal extracts used against the bed sore MRSA pathogens Isochrysis galbana extract showed highest percentage inhibition (52%) when compared to other extracts. The present study would therefore seems particularly worthy for further investigations of valuable compounds from marine microalgae.